Extracellular Matrix

Associate Professor
Department of Anatomy and Neurobiology

Northeastern Ohio Universities
College of Medicine
4209 St. Rt. 44, P.O. Box 95
Rootstown, Ohio 44272-0095
Phone: (330) 325-6304
Fax: (330) 325-5916
Email: kd@neoucom.edu

 

Education:

1988 Ph.D. Cell and Developmental Biology, Rutgers, The State University of New Jersey and University of Medicine and Dentistry of New Jersey
1981 M.S. Zoology, Rutgers, The State University of New Jersey
1977 B.A. Biology, Grinnell College


Professional Experience:

Current

 Associate Professor with tenure, Department of Anatomy and Neurobiology, Northeastern Ohio Universities College of Medicine
1994-2001 Assistant Professor, Department of Anatomy, Northeastern Ohio Universities College of Medicine
1992-1994 Research Fellow, Department of Anatomy and Cellular Biology, Tufts University School of Medicine
1992 Instructor, Department of Pathology, Robert Wood Johnson Medical School
1988-1992 Post-doctoral Fellow, Department of Pathology, Robert Wood Johnson Medical School


Research Interests:

My major research interests concentrate on the interaction of cells with the extracellular matrix using matrix receptors.  My major project elucidates the cell-matrix interactions important in heart failure fibroblasts.  The NIH grant funded as of August 1, 2005 is examining the role of collagen VI and matrix receptor signaling in transgenic type VI collagen knockout mice.  A second project, studying breast cancer, also centers around receptors and the extracellular matrix, by examining the surface proteins present on breast cancer cells at the point they begin to invade the surrounding matrix.  Two grants are currently being written on this subject, in collaboration with physicians and a researcher at Akron General Medical Center.  Another project I am involved in utilizes my expertise in immunochemistry, and involves using antibodies to detect pathogens. This project is collaborative between Kent State University (KSU; Department of Biology and the Liquid Crystal Institute) and NEOUCOM.  Three patents cover the intellectual property of this Pathogen Detection System, and we had funding from the Department of Defense to support development of the device.   We have two corporate licensees.  This project just received a Wright Development grant of $3,000,000, with an additional $3.7 million in matching funds, to develop a manufacturing facility in Ohio.  As a member of the Skeletal Biology Focus Group at NEOUCOM, I am participating in a study to examine phenotypic changes in synovial fibroblasts following arthroplasty and challenged with metals, and I am examining the role of type VI collagen in osteoarthritis.  Grants will be submitted on these projects in fall of 2008.  This group recently was awarded 8.7 million from the Ohio Research Scholar’s program to fund the development of an Orthopaedic Research Institute that included major hospitals in Akron, the University of Akron, and NEOUCOM.  Finally, I am participating in a multiple institution grant proposal (in addition to the University of Akron and Walsh University) to examine gene therapy by nanosphere delivery using a kidney hypertension model.

 

 

Selected Publications:

  • Doane, K.J., S. Howell, D. E. Birk (1998) Identification and functional characterization of two type VI collagen receptors, α3β1 and NG2, during avian corneal stromal development.  Invest. Ophthal. Vis. Sci. 39:263-275.
  • Howell, S.J., K.J. Doane (1998) Type VI collagen increases proliferation and prevents anti-β1 integrin induced-apoptosis.  Exp. Cell Res. 241:230-241.
  • Doane, K.J., R. Bhattacharya, J. Marchant. (2002) Perturbation of β1 integrin function using anti-sense or function blocking antibodies on corneal cells grown on fibronectin and tenascin: implications for corneal development.  Cell Biol. Int. 26:131-144.
  • Schneider, T., I.I. Smalyukh, T. Ishikawa, O.D. Lavrentovich, G.D. Niehaus, K.J. Doane, C.J. Woolverton (2005) Real-time microbe detection using a lyotropic chromonic liquid crystal biosensor.  Phys. Rev. E 71:020702.
  • Shiyanovskii, S.V., O.D. Lavrentovich, T. Schneider, T. Ishikawa, I.I. Smalyukh, C.J. Woolverton, G.D. Niehaus, K.J. Doane  (2005)  Lyotropic chromonic liquid crystals for biological sensing application.  Mol. Cryst. Liq. Cryst. 434:587-598.
  • Naugle, J.E., E.R. Olson, X. Zhang, S. Mase, K.J. Doane, J.G. Meszaros (2006) Type VI collagen regulation of cardiac remodeling.  Am. J. Physiol. Heart Circ. Physiol. 290:H323-H330.
  • Jenkins, C., A. Milsted, K. Doane, G. Meszaros, J. Toot, D. Ely  (2007)  A cell culture model using rat coronary artery adventitial fibroblasts to measure collagen production.  Cardiovascular Disorders 7:13-22, 2007.
  • Naugle, J.E., E.R. Olson, J.C. Koshy, D.J. Costic, M.V. Mohile, M. Dockry, K.J. Doane, J.G. Meszaros (2008)  Temporal changes in type VI collagen, myofibroblasts content, and integrin receptors post-myocardial infarction in rats.  Submitted for publication.
  • Nugent, A.E., D.M. Speicher, I. Gradisar, D.L. McBurney, A. Baraga, K.J. Doane, W.E. Horton, Jr. (2008) ER stress in advanced osteoarthritis is associated with altered collagen VI expression.  Submitted for publication.