Guinea Pig  |  Mouse  |  Rat

Mice are routinely evaluated serologically for cilia-associated respiratory bacillus (CARB), epizootic diarrhea of infant mice (EDIM), lymphocytic choriomeningitis virus (LCM), Mycoplasma pulmonis, GDVII (a strain of Theiler's encephalomyelitis virus), mouse hepatitis virus (MHV), pneumonia virus of mice (PVM), Sendai virus, mouse parvovirus (MPV), and the mouse minute virus (MMV). Once per year mice are also evaluated serologically for Ectromelia virus, Mouse adenovirus-1 and -2, Polyoma virus, Reovirus, Mouse cytomegalovirus, and Murine norovirus.  Pooled fecal samples from the sentinels in each housing room are evaluated by fecal flotation exam, and perianal tape tests from individual animals are examined for evidence of intestinal parasites. Additional testing is noted in “Comments”. Breeding colonies are tested quarterly and non-breeding colonies are tested semiannually. Interpretation of results follow the chart. 

Caging Code:

C – Conventional Shoe Box
H – Hanging
SSM – Sterile Static Microisolation
NSM – Nonsterile Static Microisolation
SVM – Sterile Ventilated Microisolation
NVM – Nonsterile Ventilated Microisolation
Sp – Special (see Comments)

Breeding: Y – Yes; N – No.  

MOUSE HEALTH MONITORING RESULTS 

Date

Room No.

Caging

Breeding

Results

Comments

May/08

  V-141 

  C 

  Y 

All evaluations were negative for evidence of pathogens. 

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease).   In April 2008 an athymic mouse was evaluated by PCR for the presence of Corynebacterium bovis and found to be negative.

 V-142

 C

 Y

 All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon
cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease).

 

 V-143

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

 V-144

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

 V-160 K

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon  cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

 V-170, Unit #7

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon  cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

 V-172, Unit #15

 C

 N

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease).  Mice acquired from a nontraditional source were quarantined and tested for all of the mouse pathogens listed in the table above (including intestinal parasites) except Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme.  The results were negative and the animals were released from quarantine. 

Feb/08 

  V-141 

  C 

  Y 

All evaluations were negative for evidence of pathogens. 

 

 V-142

 C

 Y

 All evaluations were negative for evidence of pathogens.

 

 V-143

 C

 Y

All evaluations were negative for evidence of pathogens.

 

 V-144

 C

 Y

All evaluations were negative for evidence of pathogens.

 

 V-160 K

 C

 Y

All evaluations were negative for evidence of pathogens.

 

 V-170, Unit #7

 C

 Y

All evaluations were negative for evidence of pathogens.

 

Nov/07 

V-142

 C 

Y 

All evaluations were negative for evidence of pathogens. 

Mice were also tested for Murine Norovirus. 

V-143 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-144 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-160 I 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-160 K 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-170, Unit #7 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-172, Unit #15 

C 

N 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-180 C 

SSM 

N 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

Date

Room No.

Caging

Breeding

Results

Comments

Aug/07 

V-140 

C 

Y 

All evaluations were negative for evidence of pathogens. 

 

V-141 

C 

Y 

All evaluations were negative for evidence of pathogens.

 

V-142 

C 

Y 

All evaluations were negative for evidence of pathogens.

V-142:  Mice were obtained form a nontraditional vendor in April 2007 and were maintained in a Horsfal isolation cubicle through July underwent routine pathogen testing plus Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus in July.  All tests were negative and the animals were released into the general population upon receipt of the results. 

V-143 

C 

Y 

All evaluations were negative for evidence of pathogens.

 

V-144 

C 

Y

All evaluations were negative for evidence of pathogens.

 

V-160 K 

C 

Y 

All evaluations were negative for evidence of pathogens.

 

V-170, Unit #7 

C 

Y 

All evaluations were negative for evidence of pathogens.

 

Date

Room No.

Caging

Breeding

Results

Comments

May/07 

V-140 

C

Y 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus. 

 V-141
C

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-142

C

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-143

C

Y 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-144

C

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-160 I

C

N 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-160 K

C

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-170, #7

C

Y 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-170, #16

C

N 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-180 C

SSM

N

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

Date

Room No.

Caging

Breeding

Results

Comments

Feb/07

V-140

 C

 Y 

Negative for all tests.

 

 V-141

 C

 Y

Negative for all tests.

 V-142

 C

 Y

Negative for all tests.

 

 V-143

 C

 Y

Negative for all tests.

 

 V-144

 C

 Y

Negative for all tests.

 

 V-160 I

 C

 Y

Negative for all tests.

 

 V-160 K

 C

 Y

Negative for all tests.

Date

Room No.

Caging

Breeding

Results

Comments

Nov/06 

V-140 

C 

 Y
 

Negative for all tests. 

 

 V-141

C

 Y

Negative for all tests. 

 

 V-142

C

 Y

 Negative for all tests.

 

 V-143

C

 Y

 Negative for all tests.

 

 V-144

C

 Y

 Negative for all tests.

 

 V-160 I

C

 Y

 Negative for all tests.

 

 V-160 K

C

 Y

Negative for all tests. 

One mouse in V-160 K was also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse cytomegalovirus.  All tests were negative. 

 V-170, #7

C

 Y

 Negative for all tests.

 

 V-172, #16

C

 N

 Negative for all tests.

 

 V-172, #21

C

 N

Negative for all tests. 

 

 V-180 C

SSM

 N

 Negative for all tests.

 

Date

Room No.

Caging

Breeding

Results

Comments

Aug/06 

V-141

C 

Y 

Negative for all tests. 

 

V-142

C 

Y

 Negative for all tests.

 

 V-143

C 

Y

 Negative for all tests.

 

 V-144

C 

Y

 Negative for all tests.

 

 V-160 I

C 

Y

 Negative for all tests.

 

 V-160 K

C 

Y

 Negative for all tests.

 

 V-180 C

SSM 

N

 Negative for all tests.

Although V-180 C does not contain breeding animals, no other mice were housed there and the sentinels were evaluated to preclude any potential transmission of pathogens from them to new arrival animals. 

Date

Room No.

Caging

Breeding

Results

Comments

May/06 

V-140

C 

Y 

Negative for all tests. 

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus. 

V-141 

C 

Y 

Negative for all tests.    

Same as stated for V-140.

V-142 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-143 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-144 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-160 I 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-160 J 

C 

N 

 Negative for all tests.

 Same as stated for V-140.

V-160 K 

C 

Y 

 Negative for all tests.

 Same as stated for V-140.

V-170, #6

C 

Y 

 Negative for all tests.

 Same as stated for V-140.

V-172, #17

C 

N 

 Negative for all tests.

 Same as stated for Room V-140.

V-172, #21

C 

N 

 Negative for all tests.

 Same as stated for Room V-140.

V-180 C 

SSM 

N 

 Negative for all tests.

 Same as stated for Room V-140.

Date

Room No.

Caging

Breeding

Results

Comments

Feb/06

 

 

 

 

 

 

V-140 

C 

Y 

Negative for all tests. 

 

V-141 

C 

Y 

Negative for all tests. 

 

V-142 

C 

Y 

Negative for all tests. 

 

V-143 

C 

Y 

Negative for all tests. 

 

V-144 

C 

Y 

Negative for all tests. 

 

V-160 I 

C 

Y 

Negative for all tests. 

 

V-160 K 

C 

Y 

Negative for all tests. 

Mice originating from a nontraditional vendor were placed in this room after quarantine (V-170, #5) and testing performed in January 2006 - All tests results were negative. 

Date

Room No.

Caging

Breeding

Results

Comments

Nov/05

 

 

 

 

 

 

 

 

 

 

V-140 

C 

Y 

Negative for all tests. 

 

V-141 

C 

Y 

Negative for all tests. 

 

V-142 

C 

Y 

Negative for all tests. 

 

V-143/V-144 

C 

Y 

Negative for all tests.  

Serum was pooled from these two rooms as the animals in V-144 originated from V-143 approximately two weeks prior to testing. 

V-160 H 

C 

N 

Negative for all tests. 

 

V-160 I 

C 

Y 

Negative for all tests. 

 

V-160 J 

C 

N 

Negative for all tests. 

 

V-170, #2 

C 

Y 

Negative for all tests. 

 

V-170, #7 

C 

Y 

Negative for all tests. 

 

V-172, #15 

C 

N 

Negative for all tests. 

 

V-172, #19 

C 

N 

Negative for all tests. 

 

Discussion of Findings:
Even though none of the serum samples that were tested were positive for Mouse Parvovirus, we cannot say that the mice in the affected rooms (V-143 and V-144) are free of the virus.  See May and August 2005 sentinel animal reports or contact the CMU for more information. 

Date

Room No.

Caging

Breeding

Results

Comments

Aug/05   

 

 

 

 

 

 

 V-140

C 

Y 

Positive for the intestinal parasite Aspicularis tetraptera.   Please contact the Attending Veterinarian for a detailed overview of these finding.

Negative for all other tests. 

V-141 

C 

Y 

Negative for all tests. 

 

V-142 

C 

Y 

Negative for all tests. 

 

V-143 

C 

Y 

Serologically positive for mouse parvovirus.  

Negative for all other tests. 

V-144 

C 

Y 

Negative for all tests. 

 

V-170, #2 

C 

Y 

Negative for all tests. 

 

V-170, #7 

C 

Y 

Negative for all tests. 

 

Discussion of Findings:
Mice housed in V-140 are being treated for Aspicularis tetraptera by the administration of Ivermectin in the drinking water (4 days on/3 days off every week for four weeks) coupled with twice weekly cage changes.  Follow up testing will be carried out upon completion of the treatment.

Mice housed in V-143:  As described previously, Mouse Parvovirus (MPV) can cause persistent infection in mice and can cause immune system dysfunction.  See sentinel animal reports dated May 2005 or contact the CMU for additional information.  Given that the predominant method of housing mice in the CMU is conventional shoebox style caging and the virus is somewhat resistant to environmental inactivation, additional attempts to eliminate the virus are not planned.  Please contact the CMU if it is necessary to house mice free of Mouse Parvovirus. 

Date

Room No.

Caging

Breeding

Results

Comments

May/05
V-140
C
Y
Negative for all tests.
 
V-141
C
Y
Negative for all tests.
 
V-142
C
Y
Positive for mouse parvovirus.
Negative for all other tests.
V-143
C
Y
Negative for all tests.
 
V-144
C
Y
Negative for all tests.
 
V-160 I
C
Y
Negative for all tests.
 
V-170, #7
C
N
Negative for all tests.
 
V-172, #17
C
N
Negative for all tests.
 
V-172, #21
C
N
Negative for all tests.
 
V-180 C
SSM
N
Negative for all tests.
 

  

 

Discussion of Findings:

Mice in room V-142 were found to be serologically positive for Mouse Parvovirus (MPV). MPV can cause persistent infection in mice and can cause immune system dysfunction. See sentinel animal reports dated May/June 2004 and August/September 2003 or contact the CMU for additional information.

The current positive result indicates that past attempts to eliminate the virus from the CMU have been unsuccessful OR the virus has been re-introduced. Given that the predominant method of housing mice in the CMU is conventional shoebox style caging and the virus is somewhat resistant to environmental inactivation, additional attempts to eliminate the virus from the general population appear unwise. Please contact te CMU if it is necessary to house mice free of Mouse Parvovirus.

Investigators in the CMU were notified of the positive MPV result by email on May 24, 2005.

Effective this quarter, the CMU is conducting a more complete serologic panel (Mouse Level II Profile as offered by BioReliance) on mice once yearly. This quarter mice were tested serologically for Epizootic Diarrhea of Infant Mice, GDVII virus, Mouse Hepatitis Virus, Minute Virus of Mice, Mycoplasma pulmonis, Mouse Parvovirus, Pneumonia Virus of Mice, Sendai virus, CAR Bacillus, Lymphocytic Choriomeningitis virus, Ectromelia virus, Mouse Adenovirus-1, Mouse Adenovirus-2, Mouse Cytomegalovirus, Polyoma virus, and Reovirus.

Date

Room No.

Caging

Breeding

Results

Comments

Feb/05

V-140

C

Y

Negative for all tests.

 

V-141

C

Y

Negative for all tests.

 

V-142

C

Y

Negative for all tests.

 

V-143

C

Y

Negative for all tests.

 

V-144

C

Y

Negative for all tests.

 

V-160I

C

Y