Date

Room No.

Caging

Breeding

Results

Comments

Aug/08

V-142

C

Y

All evaluations were negative for evidence of pathogens. 

Only breeding colonies of animals were tested during this quarter.

V-143

C

Y

All evaluations were negative for evidence of pathogens. 

Only breeding colonies of animals were tested during this quarter.

V-144

C

Y

Animals were found to be positive for the Murine Norovirus.

The animals housed in this room have been placed in permanent quarantine.

Only breeding colonies of animals were tested during this quarter.

V-160 K

C

Y

All evaluations were negative for evidence of pathogens. 

Only breeding colonies of animals were tested during this quarter.

V-160 M

C

Y

All evaluations were negative for evidence of pathogens. 

Only breeding colonies of animals were tested during this quarter.

V-170, Unit #7

C

Y

All evaluations were negative for evidence of pathogens. 

Only breeding colonies of animals were tested during this quarter.

Discussion of Findings:  MNV is a relatively recently identified (2003) mouse pathogen belonging to the Caliciviridae family.  It ahs been associated with clinical disease (up to and including death) and persistent infection in immunodeficient mice, especially those lacking interferon receptors.  In immunocompetent mice, asymptomatic transient infection with seroconversion appears to be the typical disease course.

Although other noroviruses infect other species, the MNV appears to be mouse specific.  Transmission of the virus can occur through the fecal-oral route, and it is inactivated by a 1:10 dilution of bleach.  Several types have been identified.  Additional information about the virus continues to appear in the literature.

At the present time, the Comparative Medicine Unit (CMU) has no plans to eliminate the virus.  Please contact the CMU if it is necessary to maintain your animals free of MNV.

May/08

V-141 

  C 

  Y 

All evaluations were negative for evidence of pathogens. 

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease).   In April 2008 an athymic mouse was evaluated by PCR for the presence of Corynebacterium bovis and found to be negative.

 V-142

 C

 Y

 All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon
cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease).

 V-143

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

 V-144

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

 V-160 K

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon  cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

V-170, Unit #7

 C

 Y

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon  cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease). 

V-172, Unit #15

 C

 N

All evaluations were negative for evidence of pathogens.

A different diagnostic laboratory was used for the serological evaluations.  The comprehensive testing performed once yearly was conducted.  In addition to performing the standard tests listed in the summary description above, mice were also tested for Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme (etiology of Tyzzer's disease).  Mice acquired from a nontraditional source were quarantined and tested for all of the mouse pathogens listed in the table above (including intestinal parasites) except Encephalitozoon cuniculi, Parvo NS-1, and Clostridium piliforme.  The results were negative and the animals were released from quarantine. 

Feb/08 

V-141 

  C 

  Y 

All evaluations were negative for evidence of pathogens. 

 V-142

 C

 Y

 All evaluations were negative for evidence of pathogens.

 V-143

 C

 Y

All evaluations were negative for evidence of pathogens.

 V-144

 C

 Y

All evaluations were negative for evidence of pathogens.

 V-160 K

 C

 Y

All evaluations were negative for evidence of pathogens.

V-170, Unit #7

 C

 Y

All evaluations were negative for evidence of pathogens.

Nov/07 

V-142

 C 

Y 

All evaluations were negative for evidence of pathogens. 

Mice were also tested for Murine Norovirus. 

V-143 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-144 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-160 I 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-160 K 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-170, Unit #7 

C 

Y 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-172, Unit #15 

C 

N 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

V-180 C 

SSM 

N 

All evaluations were negative for evidence of pathogens.

Mice were also tested for Murine Norovirus.

Date

Room No.

Caging

Breeding

Results

Comments

Aug/07 

V-140 

C 

Y 

All evaluations were negative for evidence of pathogens. 

V-141 

C 

Y 

All evaluations were negative for evidence of pathogens.

V-142 

C 

Y 

All evaluations were negative for evidence of pathogens.

V-142:  Mice were obtained form a nontraditional vendor in April 2007 and were maintained in a Horsfal isolation cubicle through July underwent routine pathogen testing plus Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus in July.  All tests were negative and the animals were released into the general population upon receipt of the results. 

V-143 

C 

Y 

All evaluations were negative for evidence of pathogens.

V-144 

C 

Y

All evaluations were negative for evidence of pathogens.

V-160 K 

C 

Y 

All evaluations were negative for evidence of pathogens.

V-170, Unit #7 

C 

Y 

All evaluations were negative for evidence of pathogens.

Date

Room No.

Caging

Breeding

Results

Comments

May/07 

V-140 

Y 

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus. 

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-142

C

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-143

C

Y 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-144

C

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-160 I

C

N 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-160 K

C

Y

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-170, #7

C

Y 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-170, #16

C

N 

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

 V-180 C

SSM

N

Negative for all tests.

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus.

Date

Room No.

Caging

Breeding

Results

Comments

Feb/07

V-140

 C

 Y 

 V-141

 C

 Y

 V-142

 C

 Y

 V-143

 C

 Y

 V-144

 C

 Y

 V-160 I

 C

 Y

 C

 Y

Date

Room No.

Caging

Breeding

Results

Comments

Nov/06 

V-140 

C 

Negative for all tests. 

 V-141

C

 Y

Negative for all tests. 

 V-142

C

 Y

 Negative for all tests.

 V-143

C

 Y

 Negative for all tests.

 V-144

C

 Y

 Negative for all tests.

 V-160 I

C

 Y

 Negative for all tests.

 V-160 K

C

 Y

Negative for all tests. 

One mouse in V-160 K was also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse cytomegalovirus.  All tests were negative. 

 V-170, #7

C

 Y

 Negative for all tests.

 V-172, #16

C

 N

 Negative for all tests.

 V-172, #21

C

 N

Negative for all tests. 

 V-180 C

SSM

 N

 Negative for all tests.

Date

Room No.

Caging

Breeding

Results

Comments

Aug/06 

C 

Y 

Negative for all tests. 

C 

 Negative for all tests.

 V-143

C 

Y

 Negative for all tests.

 V-144

C 

Y

 Negative for all tests.

 V-160 I

C 

Y

 Negative for all tests.

 V-160 K

C 

Y

 Negative for all tests.

 V-180 C

SSM 

N

 Negative for all tests.

Although V-180 C does not contain breeding animals, no other mice were housed there and the sentinels were evaluated to preclude any potential transmission of pathogens from them to new arrival animals. 

Date

Room No.

Caging

Breeding

Results

Comments

May/06 

V-140

C 

Y 

Negative for all tests. 

Mice were also tested for Ectromelia virus, Mouse adenovirus-1, Mouse adenovirus-2, Polyoma virus, Reovirus, and Mouse Cytomegalovirus. 

V-141 

C 

Y 

Negative for all tests.    

Same as stated for V-140.

V-142 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-143 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-144 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-160 I 

C 

Y 

Negative for all tests. 

Same as stated for V-140.

V-160 J 

C 

N 

 Negative for all tests.

 Same as stated for V-140.

V-160 K 

C 

Y 

 Negative for all tests.

 Same as stated for V-140.

V-170, #6

C 

Y 

 Negative for all tests.

 Same as stated for V-140.

V-172, #17

C 

N 

 Negative for all tests.

 Same as stated for Room V-140.

V-172, #21

C 

N 

 Negative for all tests.

 Same as stated for Room V-140.

V-180 C 

SSM 

N 

 Negative for all tests.

 Same as stated for Room V-140.

Date

Room No.

Caging

Breeding

Results

Comments

Feb/06

V-140 

C 

Y 

Negative for all tests. 

V-141 

C 

Y 

Negative for all tests. 

V-142 

C 

Y 

Negative for all tests. 

V-143 

C 

Y 

Negative for all tests. 

V-144 

C 

Y 

Negative for all tests. 

V-160 I 

C 

Y 

Negative for all tests. 

V-160 K 

C 

Y 

Negative for all tests. 

Mice originating from a nontraditional vendor were placed in this room after quarantine (V-170, #5) and testing performed in January 2006 - All tests results were negative. 

Date

Room No.

Caging

Breeding

Results

Comments

Nov/05

V-140 

C 

Y 

Negative for all tests. 

V-141 

C 

Y 

Negative for all tests. 

V-142 

C 

Y 

Negative for all tests. 

V-143/V-144 

C 

Y 

Negative for all tests.  

Serum was pooled from these two rooms as the animals in V-144 originated from V-143 approximately two weeks prior to testing. 

V-160 H 

C 

N 

Negative for all tests. 

V-160 I 

C 

Y 

Negative for all tests. 

V-160 J 

C 

N 

Negative for all tests. 

V-170, #2 

C 

Y 

Negative for all tests. 

V-170, #7 

C 

Y 

Negative for all tests. 

V-172, #15 

C 

N 

Negative for all tests. 

V-172, #19 

C 

N 

Negative for all tests. 

Date

Room No.

Caging

Breeding

Results

Comments

Aug/05   

 V-140

C 

Y 

Positive for the intestinal parasite Aspicularis tetraptera.   Please contact the Attending Veterinarian for a detailed overview of these finding.

Negative for all other tests. 

V-141 

C 

Y 

Negative for all tests. 

V-142 

C 

Y 

Negative for all tests. 

V-143 

C 

Y 

Serologically positive for mouse parvovirus.  

Negative for all other tests. 

V-144 

C 

Y 

Negative for all tests. 

V-170, #2 

C 

Y 

Negative for all tests. 

V-170, #7 

C 

Y 

Negative for all tests. 

Discussion of Findings:
Mice housed in V-140 are being treated for Aspicularis tetraptera by the administration of Ivermectin in the drinking water (4 days on/3 days off every week for four weeks) coupled with twice weekly cage changes.  Follow up testing will be carried out upon completion of the treatment.

Date

Room No.

Caging

Breeding

Results

Comments